![]() Modifications of the cutoff points of the ELISA test were obtained with technical adjustments done to detect HSV-2 antibodies by ELISA and Western blot using DBS samples.ĭried blood spots ELISA Herpes simplex virus type 2 Western blot.Ĭopyright © 2018 The Authors. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in. A 1:5 dilution was used and the incubation times were increased for the Western blot.ĩ08 DBS samples were processed and the following cutoff points were determined: negative (0-3.79), undetermined (3.8-4.6) and positive (≥4.61), with sensitivity and specificity close to 95%. October 2015 3 months after last sexual encounter (protected) with HSV2 partner, I got 2nd IgG test HSV2 increased to 3.56. The objective of current study was to evaluate the use of DBS samples to detect HSV-2 antibodies using commercial ELISA and Western blot tests. Summer 2015 had PROTECTED vaginal only sex about 1/2 a dozen times over a 2-month period with a HSV2 positive partner. HSV-2 is the main cause of genital ulcers and is diagnosed mainly with serological tests. Western Blot Assay: In Western blot assays, the. People want to obtain the western blot to get the most. The assay showed 100 sensitivity and 89 specificity, as confirmed by the viral culture of the same sample. ELISA was performed with 100μl and the Western blot with 200μl of eluted DBS. Background and aims: Herpes simplex virus type 2 (HSV-2) is a sexually transmitted agent and is detected worldwide. Herpes experts agree that the herpes western blot is by far the best confirmatory antibody test. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9. The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. Samples were processed by both methods to determine ELISA cutoff points using ROC curves. During the test, a small sample of blood is taken and it is used to detect HIV antibodies, not the HIV virus itself. IgG-G2 ELISA (Human ® Diagnostics, Germany) and Western blot IgG/IgM (EUROLINE-WB, Euroimmun ® Germany) tests were modified to use DBS samples. The objective of current study was to evaluate the use of DBS samples to detect HSV-2 antibodies using commercial ELISA and Western blot tests. Herpes simplex virus type 2 (HSV-2) is a sexually transmitted agent and is detected worldwide.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |